女性无套免费网站在线看-女性特黄一级毛片-女神穿上情趣丝袜啪啪一整晚-女黄色片-成人在线午夜-成人在线小视频

咨詢熱線

15000266580

當前位置:首頁 >產品中心>細胞庫>大鼠正常細胞>CRL-1589 IEC-18 大鼠回腸細胞

CRL-1589 IEC-18 大鼠回腸細胞

簡要描述:CRL-1589 IEC-18 大鼠回腸細胞, ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻和培養條件

  • 產品型號:
  • 廠商性質:生產廠家
  • 更新時間:2025-05-24
  • 訪  問  量:2198

產品分類

Product Category

詳細介紹

CRL-1589 IEC-18 大鼠回腸細胞, ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規范,提供的細胞株背景清楚,提供參考文獻i優培養條件

CRL-1589 IEC-18 大鼠回腸細胞 的詳細介紹


ATCC® Number:CRL-1589™  Price:$329.00
Designations:IEC-18

Depositors:A Quaroni

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Rattus norvegicus (rat)

Morphology:epithelial


Source:Organ: ileum
Cell Type: epithelial


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Propagation:ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 95%; fetal bovine serum, 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week


Preservation:Freeze medium: culture medium 82.5%; fetal bovine serum 10%; DMSO 7.5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X



References:22491: Quaroni A, et al. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J. Cell Biol. 80: 248-265, 1979. PubMed: 88453

26096: Quaroni A, Isselbacher KJ. Cytotoxic effects and metabolism of benzo[a]pyrene and 7,12- dimethylbenz[a]anthracene in duodenal and ileal epithelial cell cultures. J. Natl. Cancer Inst. 67: 1353-1362, 1981. PubMed: 6273638





















產品咨詢

留言框

  • 產品:

  • 您的單位:

  • 您的姓名:

  • 聯系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數字),如:三加四=7
聯系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網    管理登陸
主站蜘蛛池模板: 99精品视频在线免费观看 | 三级中文字幕永久在线视频 | 久久国产精品无码网站 | 亚洲一区二区天海翼 | 99久久国产综合精品2020 | 色吊丝avav色吊丝 | 欧美大片欧美毛片大片 | 黄色三级日韩 | 欧美视频在线一区二区三区 | 成人做爰毛片免费视频 | 在线观看一级毛片免费 | 免费刺激视频 | 本道久久综合88全国最大色 | 日韩欧美亚洲每的更新在线 | 午夜mm131美女做爰视频 | 亚洲视频在线免费播放 | 中文在线com | 久久久www免费人成看片 | 免费一级特黄 欧美大片 | 美女做爰视频在线观看免费 | 免费精品久久 | 国产成人一区免费观看 | 欧美日韩精品一区二区在线线 | 亚洲激情视频网站 | 手机免费在线看毛片 | 九九热精品在线 | 一级床上爽高清播放 | 亚洲人成综合在线播放 | 大黄一级片 | 日韩无砖专区体验区 | 中文国产成人精品久久一区 | 亚洲欧美国产精品 | 加勒比一本一道在线 | 在线日韩三级 | 欧美成人中文字幕 | 午夜精品尤物福利视频在线 | 国产一级性片 | 国产精品女在线观看 | 99re久久资源最新地址 | 99久久国产综合精品网成人影院 | 国产精品国产精品国产三级普 |