女性无套免费网站在线看-女性特黄一级毛片-女神穿上情趣丝袜啪啪一整晚-女黄色片-成人在线午夜-成人在线小视频

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠正常細(xì)胞>TIB-71RAW 264.7小鼠單核巨噬細(xì)胞白血病細(xì)胞

RAW 264.7小鼠單核巨噬細(xì)胞白血病細(xì)胞

簡(jiǎn)要描述:RAW 264.7細(xì)胞,小鼠單核巨噬細(xì)胞白血病細(xì)胞 RAW 264.7小鼠單核巨噬細(xì)胞白血病細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):TIB-71
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-05-25
  • 訪  問(wèn)  量:2850

產(chǎn)品分類

Product Category

詳細(xì)介紹

RAW 264.7細(xì)胞,小鼠單核巨噬細(xì)胞白血病細(xì)胞

ATCC® Number:  TIB-71™

Designations:  RAW 264.7

Depositors:   WC Raschke

Biosafety Level: 2

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Mus musculus (mouse)

Morphology: monocyte/macrophage

RAW 264.7細(xì)胞,小鼠單核巨噬細(xì)胞白血病細(xì)胞

Source: Tissue: ascites

Strain: BALB/c

Disease: Abelson murine leukemia virus-induced tumor

Cell Type: macrophage; Abelson murine leukemia virus transformed

Cellular Products: lysozyme [1207]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: Biological response [92560]

transfection host (Roche FuGENE® Transfection Reagents)

Receptors: complement (C3) [1207]

Antigen Expression: H-2d

Age:  *****

Gender:  male

Comments: This line was established from a tumor induced by Abelson murine leukemia virus. They are negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2)This line does not secrete detectable virus particles and is negative in the XC plaque formation assay. The cells will pinocytose neutral red and will phagocytose latex beads and zymosan. They are capable of antibody dependent lysis of sheep erythrocytes and tumor cell targets. LPS or PPD treatment for 2 days stimulates lysis of erythrocytes but not tumor cell targets.Data communicated in Feb. 2007 by Dr Janet W. Hartley, indicates the expression of infectious ecotropic MuLV closely related, if not identical, to the Moloney MuLV helper virus used in the original virus inoculum. The cells also express polytropic MuLV, unsurprisingly based on the mouse passage history of the virus stocks [ PubMed 18177500].

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol: Subcultures are prepared by scraping.

For a 75 cm2 flask, remove all but 10 ml culture medium (adjust amount accordingly for other culture vessels). Dislodge cells from the flask substrate with a cell scraper; aspirate and add appropriate aliquots of the cell suspension into new culture vessels.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended

Medium Renewal: Replace or add medium every 2 to 3 days.

Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 1135: Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031

1207: Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198

32443: Denlinger LC, et al. Regulation of inducible nitric oxide synthase expression by macrophage purinoreceptors and calcium. J. Biol. Chem. 271: 337-342, 1996. PubMed: 8550583

32466: Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116

32553: Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776

32901: Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306

33046: Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609

33076: Lokuta MA, et al. Mechanisms of murine RANTES chemokine gene induction by newcatle disease virus. J. Biol. Chem. 271: 13731-13738, 1996. PubMed: 8662857

33162: Taylor MF, et al. In vitro efficacy of morpholino-modified antisense oligomers directed against tumor necrosis factor-alpha mRNA. J. Biol. Chem. 271: 17445-17452, 1996. PubMed: 8663413

92560: Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.

16173094: Hartley JW, et al. Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line. Retrovirology. 4: 5:1, 2008. PubMed 18177500.

Related Links

NCBI Entrez Search

Cell Micrograph



RAW 264.7細(xì)胞,小鼠單核巨噬細(xì)胞白血病細(xì)胞

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 性感一级毛片 | 狠狠做久久深爱婷婷97动漫 | 国产成人精品视频午夜 | 黄色免费看片网站 | 精品国产91久久久久久久 | 免费看黄网 | 91精品国产9l久久久久 | 成人黄色一级片 | 国产视频a | 97操碰| 成年人国产视频 | 中文字幕乱 | 亚洲精品一区二区久久 | 2022国产91精品久久久久久 | 久久久精品一区二区三区 | 一区二区三区成人 | 日本b站一卡二不卡 | 亚洲伊人久久综合影院2021 | 成人夜色视频网站在线观看 | 全部免费a级毛片 | 久久国产精品高清一区二区三区 | 日韩欧美一区二区三区久久 | 日韩精品观看 | 99久久国产综合精品1尤物 | 亚洲日韩中文字幕在线播放 | 99综合在线 | 精品国产欧美一区二区 | 自拍欧美日韩 | u影一族亚洲精品欧美激情 va欧美 | 欧美成人自拍 | 欧美黑人巨大最猛性xxxxx | 日韩在线视精品在亚洲 | 日本高清不卡中文字幕 | 一级特黄爽大片刺激 | 免费观看国产网址你懂的 | 美女被爆免费视频软件 | 青青草国产免费一区二区 | 久久99久久精品视频 | 性盈盈影院67194 | 国产成人精品免费久久久久 | 国产精品拍拍拍福利在线观看 |